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2013 Association of Mid-Atlantic Aquatic Biologists Workshop
Cacapon State Park
Berkeley Springs, WV

April 4-5, 2013


The 2013 Annual Meeting and Workshop for the Association of Mid-Atlantic Aquatic Biologists (AMAAB) will be held April 4-5, 2013 at Cacapon State Park near Berkeley Springs, WV. We are planning a full day of presentations on Thursday. Mini workshops will be held on Friday morning. The host for this year's workshop is the Pennsylvania Department of Environmental Protection.

The procedures for offering presentations have not changed from previous years and can be found in the AMAAB website’s “Call for Presentations” section.

Pre-registration for 2013 AMAAB Workshop should be sent by email to mbrickner@pa.gov. Be sure to reference "[your last name] AMAAB 2013" in the subject line. Please include your contact information (name, address, telephone number). You can also call me, Tony Shaw or Mark Brickner, at 717-787-5017, to register or if you have any questions.

The registration fee is $50 which includes an evening social on Thursday, April 4th. Payments are typically made onsite at the meeting and may be made by cash or personal and agency checks made payable to AMAAB. We regret that we will not be able to accept credit cards.

Onsite Registration will take place on:
Wednesday, April 3rd -- 7:00-8:30pm
Thursday, April 4th -- 8:00-9:00am

Cash or checks will be accepted at onsite registration. If your address has changed, please leave one of your business cards at the registration table so we can update our mailing list. It is important that we get an updated email address from members because we have made the transition to conducting all business by email and website postings instead of by traditional paper mailings.

There will be a continental breakfast offered on Thursday morning (coffee, Danish, juice). For those desiring a full breakfast, the restaurant will open Thursday and Friday mornings at 7:30 a.m.

Again this year we will be holding a raffle and silent auction. This has been a big success each year. So bring a little extra cash, you may win and all helps support the meeting!

We are working on putting together photo galleries of past meetings. If you have any digital photos from the 2012 meeting (or any other year) you would like to share, please send them to Katherine Hanna (khanna@dnr.state.md.us).

Check back periodically for updates. We look forward to seeing you at the workshop.


Best regards,

Tony Shaw
2013 AMAAB President


7:00 - 8:30 PM Early registration: Wednesday April 3, 2013           Make checks payable to “AMAAB”

DAY 1 AGENDA: THURSDAY, APRIL 4, 2013 Washington/Fairfax Room

7:45 - 8:30 Registration (Continental Breakfast)

08:30 - 08:45 Welcome, Logistics, AMAAB Business

8:45 Headwaters as Indicators of Stormwater Solutions
Mark Southerland, Versar, Inc


9:10 Susquehanna Smallmouth Bass Study
Josh Lookenbill, Department of Environmental Protection


9:35 The Maryland Biological Stream Survey Training and Certification Program
Dan Boward, Maryland Dept. of Natural Resources


10:00 Pennsylvania Fish Index of Biotic Integrity
Bill Botts, PA Department of Environmental Protection


10:25 - 10:40 BREAK 15 min.

10:40 Chloride Monitoring Network Design
Allison O'Hanlon, Maryland Department of the Environment


11:05 Development of a Draft Acute Chloride Criterion for Maryland Surface Water
Timothy Fox, Maryland Department of the Environment


11:30 Creation of multi-metric macroinvertebrate indices for assessing the Ohio R.
Ryan Argo, ORSANCO


11:55 - 1:30 LUNCH 1 hour 30 min

1:25 Announcements

1:30 Fishes of Nontidal Coastal Plain Streams in Delaware: A Pilot Sampling Project
Robin Tyler, DNREC


1:55 Comparing the River Continuum Concept and the Riverine Ecosystem Synthesis Using Food Chain Length
Sean Collins, U.S. EPA/Univ. of Cincinnati


2:20 An unintended ecological consequence of dam construction: the cascading top-down effect of top predator removal
Scott Stranko, MD Department of Natural Resources


2:40 SPECIAL ANNOUNCEMENT - Dustin Shull summarizing: "Fatty acid analysis of stream biofilm to improve stream quality assessment”. Contact: Dale Honeyfield; USGS honeyfie@usgs.gov for more information

2:45 BREAK 40 min

3:15 Commemorating Mike Barbour's retirement

3:25 Biological Stressor Identification Process: Addressing MD's Non-tidal Impairments
Farah Abi-Akar, Maryland Department of the Environment


3:50 2012 South River Fish Tissue Hg Concentration Data & Whole Fillet - Biopsy Comparison
Calvin Jordon, Virginia Department of Environmental Quality


4:15 A Cytogenetic and Molecular Characterization of Two Brazilian Minnow Populations
Nick Walker, Laboratório Sistemática Molecular "Beagle"; Universidade Federal de Viçosa


4:15 Legacy Sediments: Sediment/Nutrient monitoring for stream/wetland restoration in Mid-Atlantic Region
David Rider, USEPA Region III - Philly


5:00 FINAL ANNOUNCEMENTS, BUSINESS , RAFFLES, ADJOURNMENT

6:00 DOUG & DIANE WOOD - NATIVE AMERICAN POTOMAC PRESENTATION

7:00 EVENING MIXER & POSTERS

Thursday Evening Program

18:00 DOUG WOOD - NATIVE AMERICAN POTOMAC PRESENTATION


19:00 EVENING MIXER & POSTERS

1. Using R for Graphical Analysis and Data Visualization
Facilitator: Lou Reynolds, EPA Region 3 (Reynolds.Louis@epamail.epa.gov)
This class will be an introduction to the GGPLOT 2 graphing package in R. This package allows the user to get some unique looks at biological data offering everything from xy plots to faceted geometric boxplots, all designed to help better explore datasets. It can also produce beautiful presentation quality graphics. The real power though, comes in being able to automate plot making, saving time when making and remaking the same plots over and over again. This course assumes a beginner to intermediate knowledge of R. I will supply you with code to make graphs and explore data. I will not spend much time on basics like reading data into R. I will give you code that does it and I will walk you through how to change that code to work for you. For advanced R users, only come to this class if you are a GGPLOT2 novice. I will give anyone at the meeting all the code and resources you could ever want to use GGPLOT2. If you are already R savvy and prefer R commander or prefer using no front end, that is fine. If you are trying to figure out if this is for you, visit: ggplot2.org and if you want to learn a bit about R, check out http://www.statmethods.net/
Bring a laptop loaded with a current version of R. Also download and install RStudio. Both R and R Studio are free and can be found using a search engine.
(Limit: 20 Participants)

2. Continuous Instream Monitoring
Facilitators: Michael Lookenbill, (mlookenbill@pa.gov) , Dustin Shull, (dushull@pa.gov) - PaDEP; Jeff Chaplin (jchaplin@usgs.gov) USGS-PA Science Center
Continuous Instream Monitors or data sondes have become widely used to characterize surface water quality by State and Federal agencies, as well as industry and consultants. Over the past couple of years the technology of these instruments has evolved, and the ability to apply effective quality assurance and quality control measurers has also increased. The Continuous Instream Monitoring Workshop focuses on the application of different types of equipment and at least one example of an applied protocol adopted from USGS and modified by PA DEP to fit the agencies particular needs. The workshop will hit on the basics of deployment, equipment and data maintenance, reporting, and lessons learned.
Participants are welcome to bring an instream monitor/data recorder, share QA troubleshooting issues, deployment problems, and other sonde operating questions.

3. Water Quality Standards 101: a Mini-WQS Academy
Facilitators: Tom Gardner, gardner.thomas@epa.gov EPA HQ; Mark Barath, barath.mark@epa.gov EPA Region 3 Water Quality Program
Folks: Still uncertain about the finer points of Water Quality Standards and a little uncomfortable about asking someone? Don’t fret! This workshop is intended to tie up those loose ends you may have with regards to your WQS knowledge. Serving as a refresher for the more tenured biologist and an introductory presentation for the newbie, the workshop will cover topics such as: “Waters” of the US; Designated Uses; Antideg; TMDL; Biocriteria; Endangered Species Act; Aquatic Life, Human Health, N&P and Bacteria Criteria; Monitoring; and NPDES. Newer State WQ program staff are encouraged to attend.

4. Wetland and Riparian Invasive Plants: Impacts, Identification, and Control
Facilitators: Whitney Bailey, WVU Environmental Research Center/WV DNR, whitney.bailey@mail.wvu.edu; Paul J. Harmon, WV DNR, Paul.J.Harmon@wv.gov
Non-native invasive species can significantly disrupt ecosystem functionality by decreasing biodiversity, altering community composition, and increasing structural homogeneity. It is important to document occurrences of the most common and harmful invasives so that existing populations can be managed appropriately and new ones controlled or eradicated before they cause significant harm. This presentation will give participants an overview of non-native invasive plant species that they are likely to encounter in riparian and wetland areas in the Mid-Atlantic, with a particular focus on species found in or near West Virginia. Extensive visuals will be supplemented with herbarium specimens and handouts.
A hand lens is recommended but not essential.

5. Identification and Taxonomy of Mussels
Facilitators: Dr. Art Bogan; North Carolina State Museum of Natural Sciences, (arthur.bogan@ncdenr.gov) Rick Spear; Pa-DEP (rspear@pa.gov)
The aquatic molluscan fauna of North America, north of Mexico, was historically the most diverse molluscan fauna in the world. Today, freshwater mussels (Family: Unionidae) are ranked among the most imperiled fauna group worldwide, and have been identified as the most imperiled fauna group in North America. This dramatic decline can be linked to habitat alterations, loss of host fish species, and increased siltation resulting from past dam construction along major rivers. Currently, poor land use practices, urban development, and a spectrum of domestic, industrial, and agricultural pollution have disrupted the physical and chemical properties of freshwater systems, reduced habitat quality and quantity, and accelerated the decline of freshwater mussels throughout North America.
The first portion of the workshop will examine the conservation issues facing freshwater mussels, from a worldwide and EPA Region 3 perspective, and will discuss the biology, life history, and distribution of freshwater mussels. The second will focus on taxonomic identification, providing methods as well as some examples of freshwater mussels found throughout Pennsylvania, Delaware, Maryland, DC, West Virginia, and Virginia.
Participants are encouraged, but not required, to bring their own freshwater mussel keys or resource materials. Selections from keys and other handouts will be distributed. Participants may also elect to bring their own voucher specimens.
Participants should bring their own hand lenses.
(Limit: 25 participants)

6. Conservation, Ecology and Identification of Mid-Atlantic Crayfishes
Facilitators: Zachary Loughman, Ph.D., West Liberty University (zloughman@westliberty.edu)
There are more than 350 species of crayfish in North America with around ½ currently threatened with population decline or extinction. This workshop will review crayfish biology, distribution, and ecology specific to the Mid-Atlantic region. Focus will be placed on the major anatomical characteristics used to differentiate crayfish species and key concept associated with their taxonomy. Special attention will be made to learn how to differentiate between non-native and native species and discuss the history and potential future for crayfishes in the in the Mid-Atlantic region. Identification keys will be provided and attendees will have the opportunity to key out live and preserved specimens of regional crayfish species.
Although some microscopes may be available for use, participants may wish to bring their own scope, illuminating system, extension cords and power strips. Bringing your own hand lens would be suitable.
(Limit: 25 Participants)

7. Taxonomy of Mayfly Nymphs: Beyond the 4th Edition!
Facilitator: Dr. Steve Burian, Southern Connecticut University (Burians1@southernct.edu)
Since the publication of the mayfly chapter in the 4th edition of M-C-B there have been several new taxonomic studies that have required adjustments to the keys presented at that time. This workshop will focus on updates to the mayfly chapter specifically with regard to the immature, or nymphal, stage; it will provide an overview of recent changes; information on difficult to interpret characters – specifically high lighting problems in genera of the Baetidae, Heptageniidae, Ephemerellidae, and Leptophlebiidae; and assist participants in identifying (or verifying) genera they find problematic. Although the genus-level of determination will be the primary focus of the workshop, some information on species or species-groups may be presented were possible.
Attendees: Yeah, just like you were in college lab but with no university supplied equipment - Please bring: a stereo microscope; illumination source (Limited power strips will be available but you are encouraged to bring one to share);If possible, a copy of at least the mayfly chapter from the 4th edition of M-C-B : additional materials/notes will be provided, but you need the full chapter to work from; 2 small petri dishes or Syracuse-style watch glasses; 2 pairs of watch makers (or fine tip) forceps; 1 or 2 small pin probes (e.g. #3 stainless steel insect pins); 1 pair of flat feather touch forceps for moving specimens in and out of vials; 1 small inexpensive detail water color paint brush. Optional materials: label paper, waterproof pen or soft-lead pencil, extra vials, and a few paper towels.
(Limit: 20 participants)


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